In view of the simulation optimization problems of lighting environment of navigation bridge in large warship, the simulation test flow is set up, and the simulation test elements such as luminaire optical parameters, material surface optical parameters, manikin data, and navigation bridge arrangement are determined. The simulation tests of lighting environment of navigation bridge are conducted under three operation conditions including daytime marine navigation, night marine navigation, and night berthing, and the improvement measures are proposed according to the simulation test results, which can provide auxiliary decision-making for the design optimization of navigation bridge of large warship.